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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
GeneJET Whole Blood Genomic DNA Purification Mini Kit, 50 preps, 50 prep

Brand: Fermentas

₹ 16,681.00
  • Brand: Fermentas
  • Catalog No.: K0781
  • Quantity/Unit: 50 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Whole Blood Genomic DNA Purification Mini Kit, 250 preps, 250 prep

Brand: Fermentas

₹ 74,572.00
  • Brand: Fermentas
  • Catalog No.: K0782
  • Quantity/Unit: 250 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plant Genomic DNA Purification Kit, 50 preps, 50 prep

Brand: Fermentas

₹ 18,489.00
  • Brand: Fermentas
  • Catalog No.: K0791
  • Quantity/Unit: 50 preps/Kit
  • Usually Shipped in: 1-2 Weeks
CodonBiotech Southern Blotting Teaching Kit

Brand: CODON

₹ 4,500.00
  • Brand: CODON
  • Catalog No.: CBKG109-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Own Genomic DNA Extraction Teaching Kit

Brand: CODON

₹ 3,000.00
  • Brand: CODON
  • Catalog No.: CBKG105-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Fungal DNA Extraction Teaching Kit

Brand: CODON

₹ 4,000.00
  • Brand: CODON
  • Catalog No.: CBKG102-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Plant DNA Extraction Teaching Kit

Brand: CODON

₹ 4,800.00
  • Brand: CODON
  • Catalog No.: CBKG104-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Bacterial Plasmid Isolation Teaching Kit

Brand: CODON

₹ 3,200.00
  • Brand: CODON
  • Catalog No.: CBKG106-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Bacterial DNA Extraction Teaching Kit

Brand: CODON

₹ 3,000.00
  • Brand: CODON
  • Catalog No.: CBKG101-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech DNA isolation from Blood Teaching Kit

Brand: CODON

₹ 3,600.00
  • Brand: CODON
  • Catalog No.: CBKG117-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Bacterial RNA Extraction Teaching Kit

Brand: CODON

₹ 3,200.00
  • Brand: CODON
  • Catalog No.: CBKG107-10
  • Quantity/Unit: 10 Exp/Box
CodonBiotech Animal DNA Isolation Teaching Kit

Brand: CODON

₹ 3,000.00
  • Brand: CODON
  • Catalog No.: CBKG124-10
  • Quantity/Unit: 10 Exp/Box
Aura Pure Blood/serum/plasma DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-148S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Blood/serum/plasma DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 9,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-148M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Blood/serum/plasma DNA Isolation Kit, 500 Reaction

Brand: Aura Biotech

  • Brand: Aura Biotech
  • Catalog No.: ABT-148L
  • Quantity/Unit: 500 Reaction/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-149S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 9,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-149M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 500 Rxns

Brand: Aura Biotech

  • Brand: Aura Biotech
  • Catalog No.: ABT-149L
  • Quantity/Unit: 500 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-150S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 9,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-150M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 500 Rxns

Brand: Aura Biotech

  • Brand: Aura Biotech
  • Catalog No.: ABT-150L
  • Quantity/Unit: 500 Reactions/Pack
  • Availability: In stock
Aura Pure Stool DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-151S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Stool DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 9,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-151M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Stool DNA Isolation Kit, 500 Rxns

Brand: Aura Biotech

  • Brand: Aura Biotech
  • Catalog No.: ABT-151L
  • Quantity/Unit: 500 Reactions/Pack
  • Availability: In stock
Aura Pure Urine DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-152S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Urine DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 9,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-152M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Urine DNA Isolation Kit, 500 Rxns

Brand: Aura Biotech

  • Brand: Aura Biotech
  • Catalog No.: ABT-152L
  • Quantity/Unit: 500 Reactions/Pack
  • Availability: In stock
Aura Pure sputum DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 6,900.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-153S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock