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Luminometer

Luminometer is used in laboratories to measure the light intensity or optical properties of a given sample, commonly used to measure luminescence in a sample. It basically detects any fluorescence emitted by the sample and absorbance of UV / visible light. Biological labs use the property of samples to absorb and emit light to quantify them or to qualitatively analyze them. This principle is used in several cell-based assays, gene reporter studies, luciferase assays, enzyme activity assays etc. The luminometer is used in the ATP assay, wherein quantification of ATP marker by Luminometer is used to measure the size of the microbial population. ATP produced by the colony reacts with the enzyme luciferase to emit a fluorescence which is measured using a luminometer. A photomultiplier tube or PMT is used as a detector in the luminometer. As these are quite sensitive, they can detect a range of light emitted from a sample, thereby facilitating characterization and quantification of the sample. Sometimes a syringe injection port may be provided, for aiding the addition of luminescent reagents. Kinetic measurement, RLU (Relative Light Units) and dual luciferase assay are the different measurement modes available in luminometers. Sensitivity, reproducibility, and linearity are the key criteria to be considered while selecting a suitable luminometer. Models having a low background, negligible crosstalk, big dynamic range, small footprint, and innovative instrument software are ideal. A range of models of luminometers along with accessories like microplates, cuvettes, standards, and performance measuring light plates can be availed on Biomall.

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