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PCR

PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

Blocking Buffer (10% BSA in 1XTBS)-ML185-500ML

Brand: HIMEDIA

₹ 4,800.00
  • Brand: HIMEDIA
  • Catalog No.: ML185-500ML
  • Quantity/Unit: 500ml/PACK
  • Usually Shipped in: 2-3 Weeks
Blocking Buffer (1% BSA in 1XTBS)-ML186-100ML

Brand: HIMEDIA

₹ 240.00
  • Brand: HIMEDIA
  • Catalog No.: ML186-100ML
  • Quantity/Unit: 100ml/PACK
  • Usually Shipped in: 2-3 Weeks
Blocking Buffer (1% BSA in 1XTBS)-ML186-500ML

Brand: HIMEDIA

₹ 955.00
  • Brand: HIMEDIA
  • Catalog No.: ML186-500ML
  • Quantity/Unit: 500ml/PACK
  • Usually Shipped in: 2-3 Weeks
Tween 20-MB067-100ML

Brand: HIMEDIA

₹ 4,325.00
  • Brand: HIMEDIA
  • Catalog No.: MB067-100ML
  • Quantity/Unit: 100ml/PACK
  • Usually Shipped in: 2-3 Weeks
Sodium carbonate anhydrous-MB253-100G

Brand: HIMEDIA

₹ 2,490.00
  • Brand: HIMEDIA
  • Catalog No.: MB253-100G
  • Quantity/Unit: 100gm/PACK
  • Usually Shipped in: 2-3 Weeks
Sodium carbonate anhydrous-MB253-500G

Brand: HIMEDIA

₹ 4,730.00
  • Brand: HIMEDIA
  • Catalog No.: MB253-500G
  • Quantity/Unit: 500gm/PACK
  • Usually Shipped in: 2-3 Weeks
Sodium carbonate anhydrous-MB253-1KG

Brand: HIMEDIA

₹ 9,095.00
  • Brand: HIMEDIA
  • Catalog No.: MB253-1KG
  • Quantity/Unit: 1kg/PACK
  • Usually Shipped in: 2-3 Weeks
3,3,5,5-Tetramethylbenzidine-MB122-1G

Brand: HIMEDIA

₹ 2,780.00
  • Brand: HIMEDIA
  • Catalog No.: MB122-1G
  • Quantity/Unit: 1gm/PACK
  • Usually Shipped in: 2-3 Weeks
3,3,5,5-Tetramethylbenzidine-MB122-5G

Brand: HIMEDIA

₹ 8,235.00
  • Brand: HIMEDIA
  • Catalog No.: MB122-5G
  • Quantity/Unit: 5gm/PACK
  • Usually Shipped in: 2-3 Weeks
ELISA plates-EP2-5X10No

Brand: HIMEDIA

₹ 7,995.00
  • Brand: HIMEDIA
  • Catalog No.: EP2-5X10No
  • Quantity/Unit: 5x10No/PACK
  • Usually Shipped in: 2-3 Weeks
ELISA plates-EP2-10X10No

Brand: HIMEDIA

₹ 14,810.00
  • Brand: HIMEDIA
  • Catalog No.: EP2-10X10No
  • Quantity/Unit: 10x10 No/PACK
  • Usually Shipped in: 2-3 Weeks
ELISA plates-EP2-20X10No

Brand: HIMEDIA

₹ 27,425.00
  • Brand: HIMEDIA
  • Catalog No.: EP2-20X10No
  • Quantity/Unit: 20x10 No/PACK
  • Usually Shipped in: 2-3 Weeks
Polypropylene sealing film-PR21-1X100No

Brand: HIMEDIA

₹ 9,095.00
  • Brand: HIMEDIA
  • Catalog No.: PR21-1X100No
  • Quantity/Unit: 1x100 No/PACK
  • Usually Shipped in: 2-3 Weeks
M-MuLV Reverse Transcriptase (RNase H-)-MBT072-1000U

Brand: HIMEDIA

₹ 1,660.00
  • Brand: HIMEDIA
  • Catalog No.: MBT072-1000U
  • Quantity/Unit: 1000u/Pack
  • Usually Shipped in: 2-3 Weeks
M-MuLV Reverse Transcriptase (RNase H-)-MBT072-5000U

Brand: HIMEDIA

₹ 7,395.00
  • Brand: HIMEDIA
  • Catalog No.: MBT072-5000U
  • Quantity/Unit: 5000u/Pack
  • Usually Shipped in: 2-3 Weeks
AMV Reverse Transcriptase-MBT073-100U

Brand: HIMEDIA

₹ 4,510.00
  • Brand: HIMEDIA
  • Catalog No.: MBT073-100U
  • Quantity/Unit: 100u/Pack
  • Usually Shipped in: 2-3 Weeks
AMV Reverse Transcriptase-MBT073-250U

Brand: HIMEDIA

₹ 10,800.00
  • Brand: HIMEDIA
  • Catalog No.: MBT073-250U
  • Quantity/Unit: 250u/Pack
  • Usually Shipped in: 2-3 Weeks
PCR Enhancer I-MBT085-1VL

Brand: HIMEDIA

₹ 1,445.00
  • Brand: HIMEDIA
  • Catalog No.: MBT085-1VL
  • Quantity/Unit: 1VL/Pack
  • Usually Shipped in: 2-3 Weeks
PCR Enhancer I-MBT085-5VL

Brand: HIMEDIA

₹ 6,490.00
  • Brand: HIMEDIA
  • Catalog No.: MBT085-5VL
  • Quantity/Unit: 5VL/Pack
  • Usually Shipped in: 2-3 Weeks
PCR Enhancer II-MBT086-1VL

Brand: HIMEDIA

₹ 2,035.00
  • Brand: HIMEDIA
  • Catalog No.: MBT086-1VL
  • Quantity/Unit: 1VL/Pack
  • Usually Shipped in: 2-3 Weeks
PCR Enhancer II-MBT086-5VL

Brand: HIMEDIA

₹ 7,450.00
  • Brand: HIMEDIA
  • Catalog No.: MBT086-5VL
  • Quantity/Unit: 5VL/Pack
  • Usually Shipped in: 2-3 Weeks
2X PCR TaqMixture-MBT061-100R

Brand: HIMEDIA

₹ 4,450.00
  • Brand: HIMEDIA
  • Catalog No.: MBT061-100R
  • Quantity/Unit: 100r# (2.5ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Chrom PCR Master Mix-MBT089-20R

Brand: HIMEDIA

₹ 1,700.00
  • Brand: HIMEDIA
  • Catalog No.: MBT089-20R
  • Quantity/Unit: 20r# (0.5ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Chrom PCR Master Mix-MBT089-50R

Brand: HIMEDIA

₹ 3,400.00
  • Brand: HIMEDIA
  • Catalog No.: MBT089-50R
  • Quantity/Unit: 50r# (1.25ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Chrom PCR Master Mix-MBT089-100R

Brand: HIMEDIA

₹ 6,150.00
  • Brand: HIMEDIA
  • Catalog No.: MBT089-100R
  • Quantity/Unit: 100r# (2.5ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Temp PCR Master Mix-MBT119-20R

Brand: HIMEDIA

₹ 17,500.00
  • Brand: HIMEDIA
  • Catalog No.: MBT119-20R
  • Quantity/Unit: 20r# (0.5ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Temp PCR Master Mix-MBT119-50R

Brand: HIMEDIA

₹ 35,000.00
  • Brand: HIMEDIA
  • Catalog No.: MBT119-50R
  • Quantity/Unit: 50r# (1.25ml)/pack
  • Usually Shipped in: 2-3 Weeks
Hi-Temp PCR Master Mix-MBT119-100R

Brand: HIMEDIA

₹ 64,000.00
  • Brand: HIMEDIA
  • Catalog No.: MBT119-100R
  • Quantity/Unit: 100r# (2.5ml)/pack
  • Usually Shipped in: 2-3 Weeks