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Protein Electrophoresis Buffers

Protein Electrophoresis Buffers

Protein electrophoresis is a separation technique wherein, under electric current proteins in sample gets separated as distinct bands based on their size and charge. The buffers in electrophoresis provide ions which carry a current, while also maintaining the pH. SDS-PAGE systems utilize a discontinuous buffer system to enhance the sharpness of bands in the gel. A number of buffer solutions can be used depending on pH requirement, for different steps of electrophoresis. These include transfer buffers, Tris, MOPS, MES-SDS running buffers, protein loading buffers, blocking and stripping buffers etc. Electrophoresis buffers from different brands such as HiMedia, Fisher BioReagents, Thermofisher Pierce are available to buy online on Biomall.in.
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