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Protein Purification  Kits

Protein Purification Kits

Protein purification is the process of extraction of proteins while excluding cellular components and other contaminants. The purification processes may involve several different principles such as differences in protein size, physicochemical properties, binding affinity and biological activity. Initially, the extraction process may be carried out by repeated freezing and thawing, homogenization by high pressure, lysis using detergents or enzymes, or other techniques. Following this, the cell debris is separated from the proteins and other soluble cell components by centrifugation.
There are several different purification processes and techniques available to work with proteins. Initial steps include precipitation using agents such as ammonium sulfate, using detergents to dissolve cell membrane proteins. For the actual purification, there are several chromatographic techniques available such as size exclusion, ion-exchange, affinity chromatography, HPLC and so forth.
These purification protocols require a number of biochemicals, reagent solutions, and apparatus. Protocols using all of these products are standardized and developed into kits for the convenience of end users. Biomall can source and help you in acquiring protein purification kits from an array of selection, available from popular brands such as HiMedia, BD Biosciences, Pierce and so on.
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