TRI Reagent
TRI reagent is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent is a mixture of guanidine thiocyanate and phenol in a monophasic solution.
Homogenization or lysis of the tissue sample in TRI reagent dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Application
TRI Reagent has been used:
In the isolation of total RNA from various tissue samples and cells
For the lysis of monocytes
To isolate cytoplasmic RNA from SARS-CoV infected Vero cells
To isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
To prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine
Sigma-Aldrich
T9424-100ML
100mL/Bottle
50
₹ 5,616.60/ Bottle
09 Jan, 2024
Guanidine thiocyanate-500G
Guanidine thiocyanate - 500G
Application
Guanidine thiocyanate has been used:
for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation[4]
as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay[5]
for DNA extraction from cerebrospinal fluid (CSF)[6]
Chaotropic agent and strong denaturant; solubilizes cells.
593-84-0
Sigma-Aldrich
G9277
500G/Pack
50
₹ 14,690.00/ Pack
09 Jan, 2024
Scepter 3.0 Handheld Automated Cell Counter Kit
The Scepter 3.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.The Scepter cell counter’s screen displays:Cell concentrationAverage cell sizeAverage cell volumeHistogram of size or volume distributionThis system is intended for research use only and has been tested with cell types representative of those in use today.The Scepter 3.0 Cell Counter delivers rapid cell counts in a handheld easy to use format. Its on screen instructions guide you through the sampling process and in less than 30 seconds reveal data on cell concentration, average diameter, average volume and a histogram displaying population information on your culture.
Sigma-Aldrich
PHCC360KIT
1 EA/Pack
50
₹ 3,66,231.40/ Pack
09 Jan, 2024
Accutase solution
Accutase solution sterile, filtered, suitable for cell culture.
Accutase solution (in dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Features and Benefits
Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.ment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Sigma-Aldrich
A6964-100ML
100ML/Pack
50
₹ 6,471.30/ Pack
09 Jan, 2024
StableCell Trypsin Solution
StableCell trypsin is formulated as a gentle solution for cell detachment. Enzyme activity is retained when stored at 2-8°C, and our studies even show >90
Sigma-Aldrich
T2601-500ML
500ML/Pack
50
₹ 1,986.90/ Pack
09 Jan, 2024
Millex Syringe Filter Unit, PTFE, Hydrophilic, 13 mm
Millex Syringe Filter Unit, PTFE, Hydrophilic, Non-sterile
Parameter
10 mL process volume
45 °C max. temp.
6.9 bar max. inlet pressure (100 psi)
H : 21.1 mm
filter diam. : 13 mm
filtration area : 0.65 cm2
pore size : 0.2 μm pore size
Sigma-Aldrich
SLLGX13NK
100 EA/Pack
50
₹ 32,789.99/ Pack
09 Jan, 2024
RARE EARTH DOPED PHOSPHOR NANOPARTICLES
RARE EARTH DOPED PHOSPHOR NANOPARTICLES
Cd-Free phosphor nanoparticles find applications in solid state lighting, sensors, security pigments etc. Our downshifting rare earth doped fluorescent nanoparticles are based on inorganic host lattices like sulfates, phosphates and vanadates which can be excited by a UV light source with strong emission at 275 nm. The emission characteristics of these nanoparticles has been tuned with dopants such as Bismuth, Cerium, Europium, Terbium etc. and are useful for the aforementioned applications.