10X MOPS Electrophoresis Buffer
Catalog No.: ML050-4X500ML
Usually Shipped in: 2-3 Weeks
Expiry Date : 01-Jan-1970
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10X MOPS Electrophoresis Buffer, is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 7.00 to 7.05. 10X MOPS Electrophoresis Buffer is designed for use in electrophoresis of agarose gels containing RNA samples. This is a concentrated buffer and should be diluted to 1X before use. This solution contains MOPS, Sodium hydroxide, Sodium acetate and EDTA. The pH of the solution is adjusted to 7. It is advisable to store this product room temperature (15-25C). It is a low ionic strength buffer used for electrophoretic separation of formaldehyde denatured RNA on agarose gels.
MOPS is the common name for 3-(N-morpholino) propanesulfonic acid. It is a zwitterionic buffer which was developed by Good et al. in 1966. It is structurally analogous to MES and has negligible metal binding capacity. As the pKa value of MOPS is 7.20 it is an excellent buffer for many biological systems at near-neutral pH. This is a buffer of low ionic strength and prepared from highly pure nuclease free MOPS. Usage of this buffer during electrophoresis saves a lot of time as it is very convenient.