10X Tris-Glycine-SDS Gel Running Buffer-ML042-2X500ML
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Catalog No.: ML042-2X500ML
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10X Tris-Glycine-SDS Gel Running Buffer is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 8.2 to 8.4. 10X Tris-Glycine-SDS Gel Running Buffer is the most commonly used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). This buffer is used as both anode and cathode buffer. This buffer is supplied as 10X concentrated stock solution and should be diluted to 1X with deionized water before usage. 10X Tris-Glycine-SDS Gel Running Buffer is composed of 0.5 M Tris, 1.92 M Glycine, 0.5% SDS and the pH is adjusted to 8.3. It is advisable to store this product at 2-8°C.
SDS-PAGE is a technique for separating proteins based on their ability to move within an electrical current, which is a function of the length of their polypeptide chains or of their molecular weight. The most widely used gel system for separating a broad range of proteins by SDS-PAGE is the Laemmli system (1970) which uses Tris-Glycine gels comprised of a stacking gel component and the resolving gel where varying acrylamide gel percentages are used to separate the proteins based on their mass weight. This classic system uses a discontinuous buffer system where the pH and ionic strength of the buffer used for running the gel (5X Tris-Glycine-SDS Gel Running Buffer, pH 8.3) is different from the buffers used in the stacking gel (pH 6.8) and resolving gel (pH 8.8). 10X Tris-Glycine-SDS Gel Running Buffer is used as the electrophoresis buffer during SDS-PAGE for separation and analysis of protein samples.