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20X MES-SDS Running Buffer is supplied by HiMedia. It is composed of 1M MOPS, 1M Tris, 20.5mM EDTA, 2% SDS.Bis-Tris gels are polyacrylamide gels designed to give optimal separation of small to medium sized proteins under denaturing conditions. In contrast to the alkaline conditions of SDS-PAGE gels, Bis-Tris gels are acidic