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2X PFU PCR Master Mix- 1ml

2X PFU PCR Master Mix- 1ml

₹ 2,500.00 *
(0)
  • Brand: Aura Biotech
  • Catalog No.: MPE-7S
  • Quantity/Unit: 1 ml/Pack

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Pfu DNA Polymerase Mastermix (2X) is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation, and contamination.

The included Pfu DNA Polymerase is a highly purified recombinant thermostable proofreading DNA polymerase with an about ten times higher accuracy compared to Taq DNA Polymerase. The enzyme is isolated from E.coli carrying a plasmid with the cloned Pyrococcus furiosus DNA polymerase gene. Pfu DNA Polymerase catalyzes template-dependent nucleotides polymerization in the 5'-3' direction, additionally it exhibits 3'-5' exonuclease (proofreading) activity, which allows the correction of nucleotide incorporation errors. The enzyme has no 5'-3' exonuclease activity and no detectable reverse transcriptase activity.

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