5x Synta-Ab RT-PCR-mix is a ready-to-use mixture for cDNA synthesis reaction followed by quantitative and/or qualitative PCR with real-time detection of results (RT-PCR).5x Synta-Ab Taq RT-PCR-mix contains all components necessary for RT-PCR, including Synta-RT M reverse transcriptase, Synta-Ab Taq DNA polymerase, dNTP, Mg2+ and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, template, and water must be added to the reaction mixture.Inactivation of Synta-Ab Taq polymerase with antibodies allows mixtures to be prepared at room temperature. "Hot" start provides an increase in the specificity of the amplification. Dissociation of the complex and release of Synta-Ab Taq DNA polymerase is achieved by heating above 70C.QTY-25ul