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5X Tris-SDS Buffer (pH 6.8)-ML040-2X500ML

5X Tris-SDS Buffer (pH 6.8)-ML040-2X500ML

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  • Brand: HIMEDIA
  • Catalog No.: ML040-2X500ML
  • Quantity/Unit: 2X500ml/EA
  • Usually Shipped in: 2-3 Weeks

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Tris-SDS Buffer (pH 6.8) is supplied by HiMedia. Tris-SDS Buffer (pH 6.8) is used to prepare buffer for stacking gel during SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis). It forms discontinuous stacking gel with a large pore size region for concentrating protein sample before entering separating zone. This buffer is supplied as a 5X stock. It is advisable to store this product at 2-8C.

Tris-SDS Buffer (pH 6.8) is used in the preparation of separating or resolving gel mix while performing SDS-PAGE which is used for the separation of proteins through electrophoresis and it is based on the fact that charged molecules will migrate through a matrix upon application of an electrical field. The matrix for protein electrophoresis separation is polyacrylamide. SDS-PAGE uses two types of buffer systems: the continuous buffer system and the discontinuous buffer system. In the continuous buffer system, the pH of the gel matrix remains constant throughout the separation. In contrast, the discontinuous buffer system consists of a narrow layer of stacking gel (of large pore size and acidic pH) above the main separating or resolving gel matrix of alkaline pH (pH 8.8). The stacking gel concentrates the protein sample before entering the separating gel and hence enhancing resolution. SDS-PAGE with a discontinuous buffer system is the most popular electrophoresis technique used to analyze polypeptides. Tris-SDS buffer is used in protein and nucleic acid electrophoresis.

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