6M Guanidine thiocyanate is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 4.5 to 7.0. Guandinium thiocyanate is a strong chaotropic agent and a strong protein denaturant as it inactivates nucleases almost 2.5 times faster than Guanidine hydrochloride. 6M Guanidinium thiocyanate solution is a ready to use solution which can be easily used for various molecular biology applications. It is advisable to store this product at room temperature (15-25C).

Guanidinium thiocyanate is a chaotropic agent. This is also called guanidine thiocyanate because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation. 6 M Guanidinium thiocyanate solution is used as a protein denaturant in molecular biology and commonly used for the isolation of intact RNA due to its ability to inhibit RNase activity. It is also used for lysis of cells and virus particles. It prevents the activity of RNase enzymes and DNase enzymes by denaturing them. Hence, it is used in RNA and DNA extractions. In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, due to the loss of protein secondary structure.