ABsolute Blue QPCR Mix & ROX Vial
- Brand: ABgene
- Catalog No.: AB4136B
- Quantity/Unit: 1,600 x 25 µL rxns/Vial
ABsolute Blue QPCR Mix & ROX Vial is a product from ABgene, ThermoFisher Scientific. Real-time polymerase chain reaction, or quantitative PCR is used to quantify the amplification of a specific DNA sequence. The amplification is measured using fluorescent probes and reporter dyes. The reaction uses components of the conventional PCR like a thermostable DNA polymerase, dNTPs and primers. Real-time PCR is used in diagnostics, gene expression studies, SNP genotyping, etc. The ABsolute Blue QPCR Mix & ROX Vial is a product for Real Time PCR reactions. The product has the following properties:
- This master mix is a 2X master mix for probe chemistry.
- The kit includes a blue dye for better contrast. This allows easier pipetting and well identification.
- The PCR mix has a Thermo-Stat Taq hot start enzyme and this allows a higher performance, sensitivity, consistency and convenience.
- The kit is compatible with most qPCR platforms.
- The mix is available with ROX or Fluorescein passive reference.
The mix contains the following components:
- 16 x 1.25 mL ABsolute Blue QPCR Mix (2X)
- 100 L MgCl2 (1M)
- 25 L ROX Reference Dye (1 mM)
Product properties:
Product name: ABsolute Blue QPCR Mix & ROX Vial
Brand: ABgene
Catalogue number: AB4136B
Detection Method: Primer-Probe Detection
DetectionFor Use With (Equipment): Bio-Rad CFX384 Touch,BioRad CFX96,BioRad MyiQ,BioRad iCycler iQ,BioRad iQ5,Cepheid SmartCycler,Corbett RotorGene,Eppendorf MasterCycler RealPlex,MJ Chromo4,MJ Opticon,Roche LightCycler 480 System,StepOne System
PCR method: qPCR
Polymerase: Taq DNA Polymerase
Product Line: ABsolute Blue
Product Size: 1600 reactions
Sample Type (General): DNA,dsDNA
Reaction Speed: Standard
Storage: -20C
Applications:
Gene expression analysis
siRNA validation
Genotyping