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ABsolute Blue QPCR Mix & ROX Vial

ABsolute Blue QPCR Mix & ROX Vial

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  • Brand: ABgene
  • Catalog No.: AB4136B
  • Quantity/Unit: 1,600 x 25 µL rxns/Vial

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ABsolute Blue QPCR Mix & ROX Vial is a product from ABgene, ThermoFisher Scientific. Real-time polymerase chain reaction, or quantitative PCR is used to quantify the amplification of a specific DNA sequence. The amplification is measured using fluorescent probes and reporter dyes. The reaction uses components of the conventional PCR like a thermostable DNA polymerase, dNTPs and primers. Real-time PCR is used in diagnostics, gene expression studies, SNP genotyping, etc. The ABsolute Blue QPCR Mix & ROX Vial is a product for Real Time PCR reactions. The product has the following properties:

- This master mix is a 2X master mix for probe chemistry.

- The kit includes a blue dye for better contrast. This allows easier pipetting and well identification.

- The PCR mix has a Thermo-Stat Taq hot start enzyme and this allows a higher performance, sensitivity, consistency and convenience.

- The kit is compatible with most qPCR platforms.

- The mix is available with ROX or Fluorescein passive reference.

The mix contains the following components:

- 16 x 1.25 mL ABsolute Blue QPCR Mix (2X)

- 100 L MgCl2 (1M)

- 25 L ROX Reference Dye (1 mM)

Product properties:

Product name: ABsolute Blue QPCR Mix & ROX Vial

Brand: ABgene

Catalogue number: AB4136B

Detection Method: Primer-Probe Detection

DetectionFor Use With (Equipment): Bio-Rad CFX384 Touch,BioRad CFX96,BioRad MyiQ,BioRad iCycler iQ,BioRad iQ5,Cepheid SmartCycler,Corbett RotorGene,Eppendorf MasterCycler RealPlex,MJ Chromo4,MJ Opticon,Roche LightCycler 480 System,StepOne System

PCR method: qPCR

Polymerase: Taq DNA Polymerase

Product Line: ABsolute Blue

Product Size: 1600 reactions

Sample Type (General): DNA,dsDNA

Reaction Speed: Standard

Storage: -20C

Applications:

Gene expression analysis

siRNA validation

Genotyping

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