ABsolute Blue QPCR ROX Mix is a product from ABgene, ThermoFisher Scientific. Real-time polymerase chain reaction, or quantitative PCR is used to quantify the amplification of a specific DNA sequence. The amplification is measured using fluorescent probes and reporter dyes. The reaction uses components of the conventional PCR like a thermostable DNA polymerase, dNTPs and primers. Real-time PCR is used in diagnostics, gene expression studies, SNP genotyping, etc. The ABsolute Blue QPCR ROX Mix is a product for Real Time PCR reactions. The product has the following properties:

- This master mix is a 2X master mix for probe chemistry.

- The kit includes a blue dye for better contrast. This allows easier pipetting and well identification.

- The PCR mix has a Thermo-Stat Taq hot start enzyme and this allows a higher performance, sensitivity, consistency and convenience.

- The kit is compatible with most qPCR platforms.

- The mix is available with ROX passive reference.

The mix contains the following components:

- 1 x 5 mL ABsolute Blue qPCR ROX Mix (2X)

Product properties:

Product name: ABsolute Blue QPCR ROX Mix

Brand: ABgene

Catalogue number: AB4139A

Detection Method: Primer-Probe Detection

DetectionFor Use With (Equipment): 7000 System,7300 System,7700 System,7900HT System,StepOne System,StepOnePlus System

PCR method: qPCR

Polymerase: Taq DNA Polymerase

Product Line: ABsolute Blue

Product Size: Sufficient for 400 x 25 L reactions

Sample Type (General): DNA,dsDNA

Reaction Speed: Standard

Storage: -20C

Applications:

Gene expression analysis

siRNA validation

Genotyping