Acetamide Broth (Twin Pack) is supplied by Micromaster. It is available in the form of two parts, where part A is composed of colourless deliquescent crystals and part B consists of light yellow to brick red homogeneous free flowing powder. It has a pH in the range of 6.8 to 7.2. It is advisable to store this product below 30C.

Quantity provided: 100 g/pack

Acetamide Broth is recommended for confirmation Pseudomonas aeruginosa in water samples. It is formulated as per the recommendation of Standard Methods for the Examination of Water and Wastewater. Acetamide is utilized by a wide variety of non-fermenting organisms. The media contains inorganic salts and acetamide a sole carbon and nitrogen source. However very few organisms growing in the medium metabolize acetamide by the process of deamination (acrylamidase activity). This unique ability is useful in identification of various non-fermenting gram-negative organisms. This ability is shown by Pseudomonas aeruginosa, Pseudomonas aciovorans Group III (Achromobacter xylosoxidans) and Alcaligenes odorans. Acetamide deamination leads to the liberation of ammonia, which thereby increases the pH of the medium, leading to a subsequent colour change of the phenol red indicator from yellow orange to purplish red. Some strains require upto seven days to exhibit a positive reaction as they deaminate acrylamide slowly. However, only about 40% of apyocyanogenic strains of Pseudomonas aeruginosa exhibit a positive reaction. It is therefore, not advisable to rely on this test as the only criterion for identification. Phosphates in Acetamide Broth serve as buffering agents, Magnesium sulphate is a source of ions that stimulate metabolism whereas Acetamide serves as the sole nitrogen and carbon source. Sodium chloride maintains osmotic equilibrium. Phenol red is the pH indicator.