Alkaline Transfer Buffer-ML032-100ML

Alkaline Transfer Buffer-ML032-100ML

₹ 275.00 *


Catalog No.: ML032-100ML

Quantity/Unit: 100ml/EA

Usually Shipped in: 2-3 Weeks

Expiry Date : 01-Jan-1970

Min Orderable Qty : 1 Pack

Free Shipping on orders over USD10000

For lab/research use only, unless otherwise specified

*Shipping charges extra. You may have to bear duty and taxes extra as per your country.

Product Description

Alkaline Transfer Buffer, is supplied by HiMedia. It is available in the form of a colourless solution. Alkaline Transfer Buffer contains a strong base that denatures double-stranded DNA. This buffer allows the hydrolysis of the phosphodiester backbone at the sites of depurination during Southern blotting and as a result large DNAs are very efficiently transferred and covalently linked to the positively charged nylon membrane. It is advisable to store this product room temperature (15-25C). It consists of 0.4 N Sodium hydroxide and 1 M Sodium chloride.

Alkaline Transfer Buffer is mainly used during Southern blotting procedure where DNA is transferred from agarose gel to positively charged nylon membranes. This buffer comes as a ready to use solution and can be directly used in DNA transfer. Alkaline Transfer Buffer is composed of 0.4 N Sodium hydroxide and 1 M Sodium chloride.