Alkaline Transfer Buffer, is supplied by HiMedia. It is available in the form of a colourless solution. Alkaline Transfer Buffer contains a strong base that denatures double-stranded DNA. This buffer allows the hydrolysis of the phosphodiester backbone at the sites of depurination during Southern blotting and as a result large DNAs are very efficiently transferred and covalently linked to the positively charged nylon membrane. It is advisable to store this product room temperature (15-25C). It consists of 0.4 N Sodium hydroxide and 1 M Sodium chloride.

Alkaline Transfer Buffer is mainly used during Southern blotting procedure where DNA is transferred from agarose gel to positively charged nylon membranes. This buffer comes as a ready to use solution and can be directly used in DNA transfer. Alkaline Transfer Buffer is composed of 0.4 N Sodium hydroxide and 1 M Sodium chloride.