AMEs Medium w/ L-Glutamine w/o Phenol red and Sodium bicarbonate-AT121-1L

AMEs Medium w/ L-Glutamine w/o Phenol red and Sodium bicarbonate-AT121-1L

₹ 400.00 *


Catalog No.: AT121-1L

Quantity/Unit: 1lt/EA

Usually Shipped in: 2-3 Weeks

Expiry Date : 01-Jan-1970

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

AME's Medium With L-Glutamine Without Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.3 -4.9 whereas the pH with Sodium Bicarbonate is between 7.4 -8.0. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 260.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C.Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (169.00)

Magnesium sulphate anhydrous (149.27)

Potassium chloride (231.00)

Potassium dihydrogen phosphate anhydrous (68.00)

Sodium chloride (7010.00)

Amino acids

Ascorbic acid Sodium salt (17.96)

Choline chloride (0.70)

Cytidine (0.73)

D-Biotin (0.10)

D-Ca-Pantothenic acid (Hemicalcium) (0.10)

Folic acid (0.10)

Niacinamide (0.10)

Pyridoxal hydrochloride (0.10)

Riboflavin (0.01)

Thiamine hydrochloride (0.10)

myo-Inositol (27.20)

D-Glucose (1081.00)

Hypoxanthine (0.82)

Sodium pyruvate (13.33)

Thymidine (0.24)

Uridine (0.73)

AME's medium was specially formulated to maintain rabbit retina in vitro. Rabbit retina is a good model for studying relationships between function and metabolism in organized mammalian central nervous tissue. Rabbit retina offers several advantages as it is easily accessible and strong enough to remain intact during manipulations. AME's medium is formulated to closely resemble the composition of the cerebrospinal fluid that bathes the retina in vivo. Morphologic, metabolic, and electrophysiologic measurements obtained on the in vitro retinas showed that they remained in a nearly physiological state for at least 8 hours, and even after 2 days in vitro they still exhibited a high level of metabolic activity and electrical responsiveness to light. AME's medium therefore remains the medium of choice for maintaining central nervous tissue in vitro.

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