AMPLIFYME Probe One-Step Universal RT-qPCR Mix, 500 rxns supplied by BLIRT is made for reproducible and effective synthesis of first-strand cDNA and RT-PCR in a in a single tube. It proves to be most ideal for your probe based RT-PCRs that include singleplex and multiplex gene expression studies. It is a ready to-utilize 2x concentrated mix that has all necessary fundamental contents required for RT-PCR dependent on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers. It also includes Mu-MLV Reverse Transcriptase and RNase Inhibitor in separate tubes. The utilization of antibodies with high-affinity for hot-start polymerase guarantees higher specificity, by diminishing the development of of primer-dimer structures. It permits to get more extensive unique range by eliminating competition for reaction reagents which additionally prompts sensitivity and reproducibility. The buffer components are precisely optimized resulting in ideal conditions for the activity of reverse transcriptase and hot-start polymerase. Moreover the RNA is protected by RNase Inhibitor from unspecific RNases. The AMPLIFYME Probe One-Step Universal RT-qPCR Mix gives quick, exceptionally specific one-step Real-Time RT-PCR results, giving reliable outcomes across all normally utilized Real-Time PCR stages.

It has the following applications:

RT-qPCR

gene expression analysis

genetic profilling

miRNA profiling / quantification

mass screening

RNA viral pathogen detection

characterization of genetically modified organisms (GMO)

Product Specifications:

Product name - AMPLIFYME Probe One-Step Universal RT-qPCR Mix, 500 rxns

Catalog No - AM09.1-500

Brand: BLIRT

Pack size: 500 rxn

Product Features:

Versatile excellent for various PCR conditions using different Real-Time PCR instruments

Sensitive reliable detection of low copies RNA targets

Reproducible consistent amplification across a wide dynamic range

Specific precisely selected anti-Taq antibody eliminates non-specific amplification

Efficient high efficiency in multiplex reactions

Fast accurate detection of molecular targets in as fast as 40 minutes (with either two- or three-step cycling profiles)

Universal reliable detection of RNA targets from broad range of samples