Azide Dextrose Broth
Catalog No.: DM027-500g
Usually Shipped in: 1-2 Weeks
- PRODUCT DETAILS
High grade Azide Dextrose Broth is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder which has a pH in the range of 7.0 to 7.4. This product should be stored between 10-30°C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.
• Quantity provided: 500 g/pack
Enterococci are more resistant to chlorine in water, hence are better indicators of sewage pollution than Escherichia coli. Azide Dextrose Broth is recommended by APHA for enumeration of faecal Streptococci by MPN technique. Azide Dextrose Broth was initially formulated by Rothe, Mullmann and Seligmann for quantitative determination of Enterococci in water, sewage, foods and other materials suspected of contamination with sewage. When large volumes of water samples are to be examined, double strength medium is used. Turbidity in tubes indicates presence of Enterococci, however, it should be further confirmed by inoculation in Ethyl Violet Azide Broth. Azide Dextrose Broth is a highly nutritious medium due to the presence of nutrient rich peptone special, HM peptone B and dextrose. Sodium azide has a bacteriostatic effect on most Gram-negative organisms but permits growth of Gram-positive organisms such as streptococci, some strains of staphylococci and Enterococci to grow. Streptococci detected by the above media should be further identified using chemicals.