BGjb Medium Fitton-Jackson Modification With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.9 -6.5 whereas the pH with Sodium Bicarbonate is between 7.1 -7.7. The osmolality without Sodium Bicarbonate is in the range of 300.00 -340.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 360.00 -400.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Disodium hydrogen phosphate (112.00)

Magnesium sulphate anhydrous (97.68)

Potassium chloride (400.00)

Sodium acetate (50.00)

Sodium chloride (6800.00)

Sodium dihydrogen phosphate (24.34)

Amino acids

Ascorbic acid (50.00)

D-Biotin (0.20)

D-Pantothenic acid (hemicalcium) (0.20)

Folic acid (0.20)

Nicotinic acid (20.00)

Pyridoxal 5 phosphate (0.20)

Riboflavin (0.20)

Thiamine hydrochloride (4.00)

Tocopherol phosphate disodium salt (1.00)

Vitamin B12 (0.04)

myo-Inositol (0.20)

p-Amino benzoic acid (PABA) (2.00)

Choline bitartarate (90.70)

Glucose (10000.00)

Lactic acid hemicalcium (555.00)

Phenol red sodium salt (20.00)

BGjb media were originally formulated for the growth of fetal rat long bones in a chemically defined media. The original formulation developed by Biggers, Gwatkin and Judah for embryonic bone was later modified by Sylvia Fitton Jackson and enriched with the addition of amino acids and vitamins. The buffering capacity of the medium was increased with the addition of phosphates. Fitton-Jackson Modification allows growth as well as calcification of cartilaginous embryonic bone. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.