Basal Medium Eagle (BME) (Modified for Autoclaving) With Earle's salts Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.3 -4.9 whereas the pH with Sodium Bicarbonate is between 7.2 -7.8. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6800.00)

Sodium dihydrogen phosphate anhydrous (122.00)

Amino acids

Choline bitartarate (1.80)

D-Biotin (1.00)

D-Ca-Pantothenate (1.00)

Folic acid (1.00)

Nicotinamide (1.00)

Pyridoxal hydrochloride (1.00)

Riboflavin (0.10)

Thiamine hydrochloride (1.00)

i-Inositol (1.80)

D-Glucose (1000.00)

Phenol red disodium salt (6.36)

Sodium succinate (100.00)

Succinic acid (75.00)

Basal Medium is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells, developed by Harry Eagle. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa cells and mouse fibroblast L cells in culture. This medium contains Earle's balanced salts and is modified for autoclaving. Autoclavable media offer a convenient alternative to membrane sterilized liquid medium. It is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. L-glutamine is heat labile, hence has been omitted from the formulation. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.