Brilliant Green Agar, Phenol Red, Monohydrate, Sucrose Agar Modified
Catalog No.: DM044E-500g
Availability: In stock
Expiry Date : 03-Dec-2019
- PRODUCT DETAILS
High grade Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Modified) is supplied by Micromaster. This product is available in the form of a light yellow to light pink homogenous free flowing powder which has a pH in the range of 6.7 to 7.1. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.
• Quantity provided: 500 g/pack
Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Modified) is used for selective isolation of Salmonellae other than Salmonella Typhi from faeces, foods, dairy products etc.Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar is used as a primary plating medium for isolation of Salmonella species was first described by Kristensen et al as medium for differentiation of paratyphoid B from other Gram negative enteric bacteria. It was further modified by Kauffmann for isolation of Salmonella from stool samples. Heavy inoculum and heavily contaminated samples can be analyzed by this highly selective medium. Brilliant Green Agar is used in the microbial limits test and with novobiocin for testing food samples. HMC peptone and yeast extract supplies essential amino acids and long chains of peptides for enhanced growth. Sodium chloride maintains the osmotic equilibrium. Lactose monohydrate and sucrose are the fermentable carbohydrate sources. Phenol red serves as an acid base indicator giving yellow colour to lactose and or sucrose fermenting bacteria. This medium also contains brilliant green, which inhibits growth of majority of Gram-negative and Gram-positive bacteria. Salmonella Typhi, Shigella species, Escherichia coli, Proteus species, Pseudomonas species, and Staphylococcus aureus are mostly inhibited. Non-lactose fermenting bacteria develop white to pinkish red colonies within 18-24 hours of incubation.