High grade Brilliant Green Bile Broth 2% is supplied by Micromaster. It is available in the form of a cream to pale green homogeneous free flowing powder which has a pH of around 7.0-7.4. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

Quantity provided: 100 g/pack

Brilliant Green Bile Broth 2% is one of the most widely used medium for the detection of coliform bacteria in water, wastewater, foods, and milk and dairy products. This medium is formulated as per APHA for the presumptive identification and confirmation of coliform bacteria. Peptone serves as a source of essential nutrients. Lactose is the fermentable carbohydrate. Bile inhibits gram-positive bacteria whereas the gram-negative bacteria are inhibited by brilliant green. Production of gas from lactose fermentation is detected by incorporating inverted Durham's tube, which indicates the positive evidence of faecal coliform since non faecal coliforms growing in this medium do not produce gas. Further gas production in EC broth (M127) at 45C used as a confirmation of faecal coliform. Gram-positive spore formers may produce gas if the bile or brilliant green inhibition is weakened by reaction with food material.