Buffer Set for Ligation-1150400011730
- Brand: Bangalore GeNei
- Catalog No.: 1150400011730
- Quantity/Unit: 0.5 ml x 2/Set
- Availability: In stock
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
Buffer Set for Ligation is provided by Genei to suit the needs of ligation reactions during cloning. Ligation is the process by which two fragments of nucleic acids are joined together by the formation of a phosphodiester bond. The ligation reaction is catalysed by special enzymes called ligases with the help of a cofactor such as ATP. To provide all the requirements of a ligation reaction and to create a suitable environment , several buffers are required. GeNei provides a Buffer Set for Ligation reactions. The set contains the following :
Cohesive end ligation buffer (1X) (The buffer is given in 10X concentration)
50 mM Tris HCl (pH 7.4)
10 mM Magnesium chloride
20 mM DTT
Nuclease-free BSA 50 ug/mL
1 mM ATP
Blunt end ligation buffer (1X)
50 mM Tris HCl (pH 7.4)
10 mM Magnesium chloride
1 mM DTT
5% w/u PEG
1 mM ATP
(The buffer is given in 5X concentration)
Product specifications :
Product name : Buffer Set for Ligation
Brand: Genei
Catalogue number : 1150400011730
Storage: - 20C
Price: Rs. 1,760.00