Buffered Glucose Broth
Catalog No.: DM142BS-500g
Usually Shipped in: 1-2 Weeks
- PRODUCT DETAILS
High grade Buffered Glucose Broth (Glucose Phosphate Broth) / (MR-VP Medium) is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder with a pH in the range of 6.7 to 7.1. For maximum efficiency, it must be stored below 30°C in tightly closed container.
• Quantity provided: 500g /pack
MR-VP Medium (Glucose Phosphate Broth) is recommended for the performance of the Methyl Red and Voges-Proskauer tests to differentiate between members of the coli-aerogenes group. Methyl Red and Voges-Proskauer test are among the two various tests used in the biochemical identification of bacterial species. These tests were originally studied by Voges, Proskauer and subsequently by Clark and Lubs to differentiate between members of the coli- aerogens group. Both the tests are based on the detection of specific breakdown products of carbohydrate metabolism. MR - positive organisms produce acids, resulting in a low pH (acidic) that overcomes the phosphate buffering system and maintains an acidic environment in the medium (pH 4.2 or less). MR-negative organisms further metabolize the initial fermentation products by decarboxylation to produce neutral acetyl methylcarbinol (acetoin), which results in decreased acidity in the medium and raises the pH towards neutrality (pH 6.0 or above). In the presence of atmospheric oxygen and alkali, the neutral end products, acetoin and 2, 3butanediol, are oxidized to diacetyl, which react with creatine to produce a red colour. The MR and VP tests should not be relied upon as the only means of differentiating E.coli from the Klebsiella Enterobacter groups. Occasionally, a known acetoin-positive organism fails to give a positive VP reaction