High grade Chapman Stone Agar is supplied by Micromaster. It is available in the form of a cream to yellow coarse free flowing powder which has a pH in the range of 6.8 to 7.2. It is advisable to store this product below 30C.

Quantity provided: 500 g/pack

Staphylococcus aureus is one of the most frequently isolated pathogens from clinical specimens and the most common cause of nosocomial infections. Chapman Stone Agar is a selective media used for the isolation of food poisoning staphylococci. Foods commonly contaminated with S. aureus include synthetic creams, custards and high-salted food. Chapman Stone Agar is similar to Staphylococcus Medium 110, except that the sodium chloride concentration is reduced to 5.5% and additionally ammonium sulphate is included in the formulation. The main modification consists the inclusion of ammonium sulphate in the medium that allows the direct observation of gelatin hydrolysis, instead of adding reagents to the plate medium. This medium is selective, due to the relatively high salt content, and is differential due to pigmentation, mannitol fermentation and the presence or absence of gelatin liquefaction.Casein enzymic hydrolysate, yeast extract provide nitrogen, carbon, sulphur, vitamin B and trace elements. Sodium chloride acts as a selective agent, which inhibits most of the bacterial species. Mannitol is the fermentable carbohydrate and its fermentation can be detected by adding a few drops of bromocresol purple resulting in production of yellow colour. Gelatin hydrolysis is observed as clear zones around colonies.