Chromogenic Coliform Medium with Sterile Membrane Filter is supplied by HiMedia. It contains a pale coloured filter membrane pad of 50mm diameter. Chromogenic Coliform Medium is used for the detection and enumeration of E. coli and coliforms in water samples based on chromogenic differentiation. It is advisable to store this product between 2-8C.

These are ready to use sterile culture media in the form of a 50 mm biological inert absorbent pads impregnated with standard culture medium, then dried and sterilized in 55 mm petri plate. They eliminate the need of laborious media preparation and autoclaving procedures. The nutrient pads are to be just rewetted with sterile distilled water and are ready for use. Use of nutrient pads allows larger sample volumes to be tested at a time, thereby helping time conservation. Results can be interpreted directly by counting the CFUs, therefore one can quantify the microbial load present in the sample. MF034- Chromogenic Coliform Medium is a selective medium recommended for the simultaneous detection of Escherichia coli and total coliforms in water samples. The medium contains three chromogenic substrates. The enzyme -D-galactosidase produced by coliforms cleaves 6-chloro-3-indoxyl--D-galactopyranoside to form pink to red coloured colonies. The enzyme -D-glucuronidase produced by E.coli, cleaves 5-bromo-4chloro-3-indoxyl--D-glucuronic acid. Colonies of E.coli give dark blue to violet coloured colonies due to cleavage of both the chromogens. The presence of the third chromogen IPTG enhances the colour reaction. Presence of L-Tryptophan improves the indole reaction thereby increasing the detection reliability.