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Click's Medium (EHAA) w/o L-Glutamine, Mercaptoethanol and Sodium bicarbonate

Click's Medium (EHAA) w/o L-Glutamine, Mercaptoethanol and Sodium bicarbonate

₹ 1,385.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: AT122A-5L
  • Quantity/Unit: 5lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Click's Medium (EHAA) Eagle's Hank's Amino Acids Without L-Glutamine, Mercaptoethanol and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.8 -5.4 whereas the pH with Sodium Bicarbonate is between 7.1 -7.7. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (245.53)

Disodium hydrogen phosphate anhydrous (47.90)

Magnesium sulphate anhydrous (164.90)

Potassium chloride (400.00)

Potassium dihydrogen phosphate (60.00)

Sodium chloride (8000.00)

Amino acids

Calcium pantothenate (2.00)

Choline chloride (2.00)

Folic acid (2.00)

Nicotinamide (2.00)

Pyridoxal hydrochloride (2.00)

Riboflavin (0.20)

Thiamine hydrochloride (2.00)

myo-Inositol (4.00)

Adenosine (25.00)

Cytosine (25.00)

Glucose (1000.00)

Guanosine (25.00)

Magnesium sulphate anhydrous (97.72)

Phenol red Sodium Salt (11.00)

Sodium pyruvate (275.00)

Uridine (25.00)

Click's medium was developed by Robert Click to study the in vitro immune response of mouse spleen cells. The addition of mercaptoethanol to the medium permits successful immunization of spleen cells with heterologous RBC cultured under stationary conditions without daily feeding. Click's Medium (EHAA) Eagle Hank's Amino Acids does not contain L-glutamine and mercaptoethanol. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

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