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Columbia Agar Plate-MPH144-20PT

Columbia Agar Plate-MPH144-20PT

₹ 1,535.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MPH144-20PT
  • Quantity/Unit: 20plts/Pack
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Columbia Agar Plate is supplied by HiMedia. It contains 25ml of sterile ready prepared agar in 90 mm disposable plates. This light amber coloured medium has a pH in the range of 7.1 to 7.5. Columbia Agar is mainly used for the selection and subculture of Clostridium sporogenes in accordance with the harmonized method of USP/EP/BP/JP/ IP. It is advisable to store this product between 15-25C. Composition (in gm/L) is as follows:

Pancreatic digest of casein (10.00)

Meat peptic digest (5.00)

Heart pancreatic digest (3.00)

Yeast extract (5.00)

Maize starch (1.00)

Sodium chloride (5.00)

Agar (15.00)

Columbia Blood Agar Base is commonly used as a general-purpose nutritious medium. It was devised by Ellner et al from Columbia University, which was further enriched by the addition of sheep blood. It can also be used for the isolation of organisms by addition of various supplements. Columbia Agar is prepared in accordance with the microbial limit testing harmonized methodology of USP/EP/BP/JP. This medium is recommended for checking the presence of Clostridium in non-sterile products like food, dietary, nutritional supplements related products. This medium contains 0.05% Agar and cysteine, which creates anaerobic conditions, thereby allowing anaerobic organisms to grow. Columbia Blood Agar contains pancreatic digest of casein, meat peptic digest, heart pancreatic digest and yeast extract which supports rapid and luxuriant growth of fastidious as well as non-fastidious organisms. Sodium chloride is used to maintain osmotic balance of medium. Maize starch acts as an energy source and also neutralizes toxic metabolites, in case it is produced. It is used in detection of Clostridia from pharmaceutical products. Gentamicin inhibits a number of contaminating gram-negative organisms and Staphylococcus species.

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