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Columbia Blood Agar Base-500g

Columbia Blood Agar Base-500g

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  • Brand: Micromaster
  • Catalog No.: DM063-500g
  • Quantity/Unit: 500g/Pack

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High grade Columbia Blood Agar Base is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder which has a pH in the range of 7.0 to 7.4. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

Quantity provided: 500 g/pack

Columbia Blood Agar Base was devised by Ellner et al. This medium contains special peptone which supports rapid and luxuriant growth of fastidious and non-fastidious organisms. This medium promotes typical colonial morphology, better pigment production and more sharply defined haemolytic reactions. The inclusion of bacitracin makes the enriched Columbia Agar Medium selective for the isolation of Haemophilus species from clinical specimens, especially from upper respiratory tract. Columbia Agar Base is used as the base for the media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. Corn starch serves as an energy source and also neutralizes toxic metabolites. Sheep blood permits the detection of haemolysis and also provides heme (X factor) which is required for the growth of many bacteria. However it is devoid of V factor (Nicotinamide adenine dinucleotide) and hence Haemophilus influenzae which needs both the X and V factors, will not grow on this medium.

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