Differential Reinforced Clostridial Broth Base is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 7.0 to 7.4. It is advisable to store this product below 30C.

Quantity provided: 500 g/pack

Differential Reinforced Clostridial Medium is used for the cultivation of Clostridia from water. Hirsch and Grinstead originally described Differential Reinforced Clostridial Medium to initiate the growth from small inoculum and get a higher Clostridial count. Later, Barnes and Ingram used the medium to develop vegetative cells in assays of Clostridium perfringens. This medium is developed for the isolation of sulphite-reducing Clostridia from food and for their enumeration in water by multiple tube method. Differential Reinforced Clostridial Broth is used to determine the count of sulphite reducing bacteria by MPN technique. Peptic digest of animal tissue, beef extract, yeast extract, starch, sodium acetate provide essential nutrients for bacterial metabolism. Glucose is the fermentable carbohydrate and serves as carbon and energy source. L-cysteine hydrochloride acts as reducing agent. Sodium sulphite and ferric citrate are added as indicators. Sulphite reducing clostridia produce sulphide from sulphite, which results in the formation of black coloured medium.