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DMEM / F12, 1:1 Mixture w/ Trace elements, L-Glutamine and NaHCO3 w/o HEPES buffer

DMEM / F12, 1:1 Mixture w/ Trace elements, L-Glutamine and NaHCO3 w/o HEPES buffer

₹ 2,585.00 *
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  • Brand: HIMEDIA
  • Catalog No.: AL127S-500ML
  • Quantity/Unit: 500ml/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) With L-Glutamine, Trace elements and 1.5gms per litre Sodium bicarbonate Without HEPES buffer, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.0005)

Ammonium molybdate tetrahydrate (0.006)

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Nickel chloride (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (1500.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate (54.30)

Sodium metasillicate nonahydrate (0.014)

Sodium selenite (0.0051)

Stannous chloride dihydrate (0.0001)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

Dulbecco's Modified Eagle Medium/Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This is a 1X Liquid Cell Culture Medium with L-glutamine, 1.5gms per litre sodium bicarbonate and trace elements. It does not contain HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

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