DMEM, High glucose w/ 4.5g/L Glucose and Sodium pyruvate w/o L-Glutamine and NaHCO3

DMEM, High glucose w/ 4.5g/L Glucose and Sodium pyruvate w/o L-Glutamine and NaHCO3

₹ 3,250.00 *

Brand: HIMEDIA

Catalog No.: AT007A-10X1L

Quantity/Unit: 10x1lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre and Sodium pyruvate Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.9 -6.5 whereas the pH with Sodium Bicarbonate is between 7.4-8.0. The osmolality without Sodium Bicarbonate is in the range of 250.00 -290.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 330.00 -370.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• D-Glucose (4500.00)

• Phenol red sodium salt (16.00)

• Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. This product contains 4.5gm glucose per litre and sodium pyruvate but does not contain L-glutamine. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

 

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