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DMEM, High glucose w/ HEPES Buffer, L-Glutamine, Sodium pyruvate w/o NaHCO3, Phenol red

DMEM, High glucose w/ HEPES Buffer, L-Glutamine, Sodium pyruvate w/o NaHCO3, Phenol red

₹ 540.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: AT187-1L
  • Quantity/Unit: 1lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Dulbecco's Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre, 25mM HEPES Buffer, L-Glutamine and Sodium pyruvate Without Sodium bicarbonate and Phenol red is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.2 -5.8 whereas the pH with Sodium Bicarbonate is between 6.6-7.2. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 355.00 -395.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (4.00)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.400)

Thiamine hydrochloride (4.00)

i-Inositol (7.20)

D-Glucose (4500.00)

HEPES buffer (5958.00)

Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. This product contains 4.5gms glucose per litre, L-glutamine, Sodium pyruvate and 25 mM HEPES Buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. This medium does not contain sodium bicarbonate and phenol red.

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