Dulbecco's Modified Eagle Medium (DMEM) With L-Glutamine, 4.5gm Glucose per litre, Sodium pyruvate and 25mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.4 -6.0 whereas the pH with Sodium Bicarbonate is between 6.7-7.3. The osmolality without Sodium Bicarbonate is in the range of 285.00 -325.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 355.00 -395.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (4.00)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.400)

Thiamine hydrochloride (4.00)

i-Inositol (7.20)

D-Glucose (4500.00)

HEPES buffer (5958.00)

Phenol red sodium salt (15.90)

Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. This product contains 4.5gms glucose per litre, L-glutamine, sodium pyruvate and 25mM HEPES Buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.