DMEM, High glucose w/ L-Glutamine, Sodium pyruvate, HEPES w/o Phenol red, NaHCO3

DMEM, High glucose w/ L-Glutamine, Sodium pyruvate, HEPES w/o Phenol red, NaHCO3

₹ 6,100.00 *

Brand: HIMEDIA

Catalog No.: AT241-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre, L-Glutamine, Sodium pyruvate and 25mm HEPES buffer Without Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder.It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• D-Glucose (4500.00)

• HEPES buffer (5958.00)

• Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

 

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