DMEM, L-Glutamine, HEPES buffer and Sodium Pyruvate w/o NaHCO3

DMEM, L-Glutamine, HEPES buffer and Sodium Pyruvate w/o NaHCO3

₹ 490.00 *


Catalog No.: AT149-1L

Quantity/Unit: 1lt/EA

Usually Shipped in: 2-3 Weeks

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) With L-Glutamine, 1gm Glucose per litre, 25mM HEPES buffer and Sodium pyruvate Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.3 -5.9 whereas the pH with Sodium Bicarbonate is between 6.7-7.3. The osmolality without Sodium Bicarbonate is in the range of 300.00 -340.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 380.00 -420.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• D-Glucose (1000.00)

• HEPES buffer (5958.00)

• Phenol red sodium salt (15.90)

• Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells. This medium contains 1gm glucose per litre, L-glutamine, sodium pyruvate and 25mM HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


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