DMEM Low glucose (Modified for Autoclaving) w/ Sodium pyruvate w/o L-Glutamine, NaHCO3

DMEM Low glucose (Modified for Autoclaving) w/ Sodium pyruvate w/o L-Glutamine, NaHCO3

₹ 3,400.00 *

Brand: HIMEDIA

Catalog No.: AT065A-10X1L

Quantity/Unit: 10x1lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) (Modified for Autoclaving) With 1gm Glucose per litre and Sodium pyruvate Without L-Glutamine and Sodium bicarbonate is supplied HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.5 -5.1 whereas the pH with Sodium Bicarbonate is between 7.6-8.2. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 290.00 -330.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• D-Glucose (1000.00)

• Phenol red sodium salt (9.00)

• Sodium pyruvate (110.00)

• Sodium succinate (100.00)

• Succinic acid (75.00)

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg glucose per litre and was originally used to culture embryonic mouse cells. This is a low glucose medium, modified for autoclaving. Autoclavable medium offers a convenient alternative to membrane sterilized liquid medium and is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. L-glutamine is heat labile, hence has been omitted from the formulation.

 

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