DMEM, Low glucose w/ 1gm Glucose per litre, L-Glutamine and Sodium pyruvate w/o NaHCO3

Dulbecco's Modified Eagle Medium (DMEM) With L-Glutamine, 1gm Glucose per litre and Sodium pyruvate Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.7 -6.3 whereas the pH with Sodium Bicarbonate is between 7.4-8.0. The osmolality without Sodium Bicarbonate is in the range of 240.00 -280.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (4.00)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.400)

Thiamine hydrochloride (4.00)

i-Inositol (7.20)

D-Glucose (1000.00)

Phenol red sodium salt (15.90)

Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg/L of Glucose and was originally used to culture embryonic mouse cells. This medium contains 1gm glucose per litre, L-glutamine & sodium pyruvate. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.