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DMEM, Low glucose w/ 1gm Glucose per litre, Sodium Pyruvate w/o L-Glutamine, NaHCO3

DMEM, Low glucose w/ 1gm Glucose per litre, Sodium Pyruvate w/o L-Glutamine, NaHCO3

₹ 8,130.00 *
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  • Brand: HIMEDIA
  • Catalog No.: AT006A-50L
  • Quantity/Unit: 50lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Dulbecco's Modified Eagle Medium (DMEM) With 1gm Glucose per litre and Sodium pyruvate Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.9 -6.5 whereas the pH with Sodium Bicarbonate is between 7.5-8.1. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (4.00)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.400)

Thiamine hydrochloride (4.00)

i-Inositol (7.20)

D-Glucose (1000.00)

Phenol red sodium salt (15.90)

Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagles medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg glucose per litre and was originally used to culture embryonic mouse cells. This medium contains 1.0gm glucose per litre and sodium pyruvate but does not contain L-glutamine. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

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