DMEM w/o L-Glutamine, Glucose, Phenol red, Sodium pyruvate and Sodium bicarbonate

DMEM w/o L-Glutamine, Glucose, Phenol red, Sodium pyruvate and Sodium bicarbonate

₹ 8,000.00 *

Brand: HIMEDIA

Catalog No.: AT063A-50L

Quantity/Unit: 50lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) Without L-Glutamine, Glucose, Phenol red, Sodium Pyruvate and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.8 -6.4 whereas the pH with Sodium Bicarbonate is between 7.4-8.0. The osmolality without Sodium Bicarbonate is in the range of 220.00 -260.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg glucose per litre and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4.5gms/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. This medium does not contain L-glutamine, glucose, phenol red, sodium pyruvate and sodium bicarbonate.

 

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