Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham(DMEM/F12, 1:1 mixture) With L-Glutamine, 15mM HEPES buffer and Trace elements Without Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.5 -6.1 whereas the pH with Sodium Bicarbonate is between 6.6 -7.2. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 300 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.0013)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Potassium chloride (311.80)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate monohydrate (54.30)

Zinc sulphate heptahydrate (0.432)

Amino acids

Ca-D-Pantothenic acid (2.24)

Choline chloride (8.98)

D-Biotin (0.0035)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.031)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic Acid (0.105)

HEPES Buffer (3754.50)

Hypoxanthine (2.40)

Linoleic acid (0.042)

Putrescine hydrochloride (0.081)

Sodium pyruvate (110.00)

Thymidine (0.365)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains L-glutamine, 15mM HEPES buffer and trace elements but does not contain phenol red and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.