WORLDWIDE SHIPPING
DMEM/F12, 1:1 Mixture w/ L-Alanyl-L-Glutamine, NaHCO3 w/o HEPES, Trace elements

DMEM/F12, 1:1 Mixture w/ L-Alanyl-L-Glutamine, NaHCO3 w/o HEPES, Trace elements

₹ 2,740.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: AL155G-500ML
  • Quantity/Unit: 500ml/EA
  • Usually Shipped in: 2-3 Weeks

- +

Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

Share:

HiGlutaXL Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) With L-Alanyl-L-Glutamine and 2.438gms per litre Sodium bicarbonate Without HEPES buffer and Trace elements is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (2438.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate (54.30)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

HiGlutaXL medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4C for longer periods. This is a 1X Liquid Cell Culture Medium with L-alanyl-L-glutamine and 2.438gms per litre sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

Related Products