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DMEM/F12, 1:1 Mixture w/ L-Glutamine, HEPES buffer, NaHCO3 w/o Phenol red, Trace elements

DMEM/F12, 1:1 Mixture w/ L-Glutamine, HEPES buffer, NaHCO3 w/o Phenol red, Trace elements

₹ 2,835.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: AL215A-500ML
  • Quantity/Unit: 500ml/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) With L-Glutamine, 15mM HEPES buffer and Sodium bicarbonate Without Phenol red and Trace elements, is supplied by HiMedia. It is available in the form of a colourless, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 290.00 -330.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Potassium chloride (311.80)

Sodium bicarbonate (1200.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate monohydrate (54.30)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

HEPES buffer (3574.50)

Hypoxanthine (2.40)

L-Glutamine (365.00)

Linoleic acid (0.04)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

Dulbeccos Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This is a 1X Liquid Cell Culture Medium with L-glutamine, sodium bicarbonate and 15mM HEPES buffer. It does not contain phenol red and trace elements elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C, prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

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