DMEM/F12, 1:1 mixture w/ L-Glutamine, HEPES Buffer w/o Trace elements, NaHCO3

DMEM/F12, 1:1 mixture w/ L-Glutamine, HEPES Buffer w/o Trace elements, NaHCO3

₹ 5,200.00 *


Catalog No.: AT140-10X1L

Quantity/Unit: 10x1lt/EA

Usually Shipped in: 2-3 Weeks

Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) With L-Glutamine and 15mM HEPES buffer Without Trace elements and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.5 -6.1 whereas the pH with Sodium Bicarbonate is between 6.5-7.1. The osmolality without Sodium Bicarbonate is in the range of 270.00 -310.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (154.50)

• Copper sulphate pentahydrate (0.0013) 

• Disodium hydrogen phosphate (71.02) 

• Ferric nitrate nonahydrate (0.05) 

• Ferrous sulphate heptahydrate (0.42) 

• Magnesium chloride hexahydrate (61.20) 

• Magnesium sulphate anhydrous (48.84) 

• Potassium chloride (311.80)

• Sodium chloride (6996.00) 

• Sodium dihydrogen phosphate monohydrate (54.30) 

• Zinc sulphate heptahydrate (0.432)

• Amino acids

• Ca-D-Pantothenic acid (2.24) 

• Choline chloride (8.98) 

• D-Biotin (0.0035) 

• Folic acid (2.66)

• Niacinamide (2.02) 

• Pyridoxal hydrochloride (2.00)

• Pyridoxine hydrochloride (0.031) 

• Riboflavin (0.22)

• Thiamine hydrochloride (2.17)

• Vitamin B12 (0.68)

• myo-Inositol (12.60)

• D-Glucose (3151.00) 

• DL-Thioctic Acid (0.105)

• HEPES Buffer (3574.50)

• Hypoxanthine (2.40) 

• Linoleic acid (0.042) 

• Phenol red sodium salt (8.63)

• Putrescine hydrochloride (0.081) 

• Sodium pyruvate (110.00) 

• Thymidine (0.365)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains L-glutamine and 15mM HEPES buffer but does not contain trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


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