DMEM/F12, 1:1 mixture w/ Trace elements w/o L-Glutamine, Phenol red, NaHCO3, HEPES buffer

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) With Trace elements Without HEPES buffer, L-Glutamine, Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.0 -6.6 whereas the pH with Sodium Bicarbonate is between 7.2-7.8. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 285.00 -325.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Ammonium metavanadate (0.00058)

• Ammonium molybdate tetrahydrate (0.0062) 

• Calcium chloride dihydrate (154.50)

• Copper sulphate pentahydrate (0.0013) 

• Disodium hydrogen phosphate (71.02) 

• Ferric nitrate nonahydrate (0.05) 

• Ferrous sulphate heptahydrate (0.42) 

• Magnesium chloride hexahydrate (61.20) 

• Magnesium sulphate anhydrous (48.84) 

• Manganese sulphate (0.000151) 

• Nickel chloride (0.00012)

• Potassium chloride (311.80)

• Sodium chloride (6996.00) 

• Sodium dihydrogen phosphate monohydrate (54.30) 

• Sodium metasillicate nonahydrate (0.014) 

• Sodium selenite (0.0052) 

• Stannous chloride dihydrate (0.00011)

• Zinc sulphate heptahydrate (0.432)

• Amino acids

• Ca-D-Pantothenic acid (2.24) 

• Choline chloride (8.98) 

• D-Biotin (0.0035) 

• Folic acid (2.66)

• Niacinamide (2.02) 

• Pyridoxal hydrochloride (2.00)

• Pyridoxine hydrochloride (0.031) 

• Riboflavin (0.22)

• Thiamine hydrochloride (2.17)

•  Vitamin B12 (0.68)

•  myo-Inositol (12.60)

• D-Glucose (3151.00) 

• DL-Thioctic Acid (0.105)

• Hypoxanthine (2.40) 

• Linoleic acid (0.042) 

• Putrescine hydrochloride (0.081) 

• Sodium pyruvate (110.00) 

• Thymidine (0.365)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains trace elements but does not contain HEPES buffer, L-glutamine and phenol red. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.