DMEM/F12, 1:1 mixture w/ Trace elements w/o L-Glutamine, Phenol red, NaHCO3, HEPES buffer
₹ 2,100.00 *
Brand: HIMEDIA
Catalog No.: AT147A-5L
Quantity/Unit: 5lt/EA
Usually Shipped in: 2-3 Weeks
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Product Description
Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) With Trace elements Without HEPES buffer, L-Glutamine, Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.0 -6.6 whereas the pH with Sodium Bicarbonate is between 7.2-7.8. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 285.00 -325.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:
• Ammonium metavanadate (0.00058)
• Ammonium molybdate tetrahydrate (0.0062)
• Calcium chloride dihydrate (154.50)
• Copper sulphate pentahydrate (0.0013)
• Disodium hydrogen phosphate (71.02)
• Ferric nitrate nonahydrate (0.05)
• Ferrous sulphate heptahydrate (0.42)
• Magnesium chloride hexahydrate (61.20)
• Magnesium sulphate anhydrous (48.84)
• Manganese sulphate (0.000151)
• Nickel chloride (0.00012)
• Potassium chloride (311.80)
• Sodium chloride (6996.00)
• Sodium dihydrogen phosphate monohydrate (54.30)
• Sodium metasillicate nonahydrate (0.014)
• Sodium selenite (0.0052)
• Stannous chloride dihydrate (0.00011)
• Zinc sulphate heptahydrate (0.432)
• Amino acids
• Ca-D-Pantothenic acid (2.24)
• Choline chloride (8.98)
• D-Biotin (0.0035)
• Folic acid (2.66)
• Niacinamide (2.02)
• Pyridoxal hydrochloride (2.00)
• Pyridoxine hydrochloride (0.031)
• Riboflavin (0.22)
• Thiamine hydrochloride (2.17)
• Vitamin B12 (0.68)
• myo-Inositol (12.60)
• D-Glucose (3151.00)
• DL-Thioctic Acid (0.105)
• Hypoxanthine (2.40)
• Linoleic acid (0.042)
• Putrescine hydrochloride (0.081)
• Sodium pyruvate (110.00)
• Thymidine (0.365)
Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains trace elements but does not contain HEPES buffer, L-glutamine and phenol red. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
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