Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) With Trace elements Without HEPES buffer, L-Glutamine, Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.0 -6.6 whereas the pH with Sodium Bicarbonate is between 7.2-7.8. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 285.00 -325.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.00058)

Ammonium molybdate tetrahydrate (0.0062)

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.0013)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.000151)

Nickel chloride (0.00012)

Potassium chloride (311.80)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate monohydrate (54.30)

Sodium metasillicate nonahydrate (0.014)

Sodium selenite (0.0052)

Stannous chloride dihydrate (0.00011)

Zinc sulphate heptahydrate (0.432)

Amino acids

Ca-D-Pantothenic acid (2.24)

Choline chloride (8.98)

D-Biotin (0.0035)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.031)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic Acid (0.105)

Hypoxanthine (2.40)

Linoleic acid (0.042)

Putrescine hydrochloride (0.081)

Sodium pyruvate (110.00)

Thymidine (0.365)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains trace elements but does not contain HEPES buffer, L-glutamine and phenol red. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.