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DMEM/F12, 3:1 Mixture w/ L-Glutamine w/o Calcium chloride, HEPES buffer, NaHCO3

DMEM/F12, 3:1 Mixture w/ L-Glutamine w/o Calcium chloride, HEPES buffer, NaHCO3

₹ 8,375.00 *
  • Brand: HIMEDIA
  • Catalog No.: AT189-20L
  • Quantity/Unit: 20lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 3:1 Mixture) With L-Glutamine Without Calcium Chloride, HEPES buffer and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.0 -6.6 whereas the pH with Sodium Bicarbonate is between 7.2 -7.8. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Copper sulphate pentahydrate (0.000625)

Disodium hydrogen phosphate anhydrous (35.51)

Ferric nitrate nonahydrate (0.07)

Ferrous sulphate heptahydrate (0.21)

Magnesium chloride anhydrous (14.41)

Magnesium sulphate anhydrous (73.29)

Potassium chloride (356.00)

Sodium chloride (6699.75)

Sodium dihydrogen phosphate anhydrous (81.75)

Zinc sulphate heptahydrate (0.21)

Amino acids

Choline chloride (6.49)

D-Biotin (0.001825)

D-Ca-Pantothenate (3.12)

Folic acid (3.33)

Niacinamide (3.00)

Pyridoxine hydrochloride (3.015)

Riboflavin (0.31)

Thiamine hydrochloride (3.08)

Vitamin B12 (0.34)

myo-Inositol (9.90)

D-Glucose (3825.50)

DL-Thioctic acid (0.05)

Hypoxanthine (1.20)

Linoleic acid (0.02)

Phenol red sodium salt (12.235)

Putrescine dihydrochloride (0.04)

Sodium pyruvate (27.525)

Thymidine (0.18)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This product contains L-glutamine but does not contain calcium chloride, HEPES buffer and sodium bicarbonate. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

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