DNaseMe (dsDNase),25000 U
- Brand: Blirt
- Catalog No.: EN33-250
- Quantity/Unit: 25000 U /bottle
DNaseMe (dsDNase),25000 U supplied by BLIRT is an endonuclease recombinant enzyme that is derived from marine amphipods (expressed in Pichia pastoris). It has a molecular weight of 42.8 kDa. DNaseMe shows high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. It is a broad spectrum enzyme that can withstand a wide range of temperatures, buffer conditions and pH. The specific activity of the enzyme is similar to bovine DNase I however, DNaseMe is characterized by higher stability in demanding reaction and storage conditions (e.g. high salt and detergent containing buffers, elevated temperature). DNaseMe hydrolyzes phosphodiester linkages yielding oligonucleotides with a 5-phosphate and a 3-hydroxyl groups and extremely useful for rapid and RNA safe degradation of genomic DNA, where absence of ribonucleases is critical to maintain the integrity of RNA.
Applications include:
Extraction and purification of RNA (equivalent of DNase I).
Removal of contaminating genomic DNA from RNA samples.
Degradation of DNA template in transcription reactions.
Reduction of viscosity in biological samples.
Removal of residual DNA during primary stem cell isolation, biopharma and bioprocessing procedures.
Product Specifications:
Product name - DNaseMe (dsDNase),25000 U
Catalog No - EN33-250
Brand: BLIRT
Pack size: 25000 U
Product Features:
Active in a broad temperature range (10 80C).
Active in a broad pH range (optimum at pH 6.0 9.0).
Highly active at elevated salt concentrations and other typical buffer additives (Table 1), which significantly improves efficiency and yield of various workflows.
Requires bivalent cations (Mg2+ and Ca2+) for maximal activity.
Degrades dsDNA to fragments below 10 nt.
The activity towards dsDNA is at least 1000 times higher than towards ssDNA
Unit definition: One unit (U) is defined as the amount of enzyme that causes an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37C and pH 8.0 with herring sperm DNA as a substrate.