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EXTRACTME DNA BLOOD KIT, 250 preps

EXTRACTME DNA BLOOD KIT, 250 preps

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  • Brand: Blirt
  • Catalog No.: EM05-250
  • Quantity/Unit: 250 preps/pack

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EXTRACTME DNA BLOOD KIT, 250 preps supplied by BLIRT is intended for a fast and effective purification of high quality (genomic, mitochondrial and viral) DNA from whole blood (fresh or frozen, human or mammalian), plasma, serum, buffy coat, lymphocytes and body fluids. The protocol for isolation and buffer formulations is optimized for high isolation efficiency and DNA purity. The process for DNA purification involves 5 steps and uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st step includes involves the lysis of red blood cells. There is no DNA in the cells and they prove to be a potential source of PCR inhibitors a this time. Due to which they should be segregated from the white blood cells prior to DNA isolation. The WBCs are then lysed by by Proteinase K in optimized BL Buffer. All the cellular membranes and proteins gets degraded in this stage. The utilization of RNase A is advised only when it is necessary to eliminate RNA. The lysate is applied to the purification minicolumns membrane post the addition of chaotropic salts and the DNA is bound. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications like PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

Product Specifications:

Product name - EXTRACTME DNA BLOOD KIT, 50 preps

Catalog No - EM05-250

Brand: BLIRT

Pack size: 250 preps

Product Features:

1 SAMPLE MATERIAL

up to 1 ml of fresh or frozen blood, up to 200 l plasma, serum, buffy coat, lymphocytes or body fluids

2. BINDING CAPACITY

Approx. 27 g DNA

3. TIME REQUIRED

25 minutes (including incubation time)

4. DNA PURITY

A260/A280 ratio = 1.7 1.9

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